klenow fragment Search Results


klenow fragment  (New England Biolabs)
99
New England Biolabs klenow fragment
Klenow Fragment, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/klenow+fragment/Klenow+Fragment/us11608527-874-24-20
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klenow fragment  (Jena Bioscience)
94
Jena Bioscience klenow fragment
A) Schematic of DNA construct preparation for single-molecule force spectroscopy. A ∼6.8 kb plasmid was linearized with the restriction enzyme NotI and the 5’-overhangs were biotinylated using <t>Klenow</t> <t>fragment.</t> The CpG methyltransferase (MTase) M.SssI was used to methylate all cytosines at the C-5 position (5mC) within the double-stranded CG dinucleotide sequence. B) Interpolation of force-extension data shows that the averaged force-distance (F,d) curves for unmethylated (−5mC) and methylated (+5mC) DNA follow different trajectories, reflecting variation in the mechanical properties upon CpG methylation. C ) CpG methylation significantly increases the DNA contour length (L C ) (Student’s t-test, p < 0.001, n = 34 and n = 26 for -5mC and +5mC, respectively). Box plots show quartiles; whiskers show the distribution excluding outliers. D ) CpG methylation significantly increases the DNA persistence length (L p ) from 47 ± 8 nm (unmethylated) to 52 ± 7 nm (methylated) (Student’s T-test, p < 0.05, n = 34 and n = 26 for -5mC and +5mC, respectively).
Klenow Fragment, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/klenow+fragment/Klenow+Fragment/bio_rxiv__64898__2026__04__13__715649-141-8-10
Average 94 stars, based on 1 article reviews
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dna polymerase i  (New England Biolabs)
98
New England Biolabs dna polymerase i
A) Schematic of DNA construct preparation for single-molecule force spectroscopy. A ∼6.8 kb plasmid was linearized with the restriction enzyme NotI and the 5’-overhangs were biotinylated using <t>Klenow</t> <t>fragment.</t> The CpG methyltransferase (MTase) M.SssI was used to methylate all cytosines at the C-5 position (5mC) within the double-stranded CG dinucleotide sequence. B) Interpolation of force-extension data shows that the averaged force-distance (F,d) curves for unmethylated (−5mC) and methylated (+5mC) DNA follow different trajectories, reflecting variation in the mechanical properties upon CpG methylation. C ) CpG methylation significantly increases the DNA contour length (L C ) (Student’s t-test, p < 0.001, n = 34 and n = 26 for -5mC and +5mC, respectively). Box plots show quartiles; whiskers show the distribution excluding outliers. D ) CpG methylation significantly increases the DNA persistence length (L p ) from 47 ± 8 nm (unmethylated) to 52 ± 7 nm (methylated) (Student’s T-test, p < 0.05, n = 34 and n = 26 for -5mC and +5mC, respectively).
Dna Polymerase I, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/klenow+fragment/DNA+Polymerase+I%2C+Large+(Klenow)+Fragment/bio_rxiv__64898__2026__03__13__711564-239-10-14
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klenow fragment  (Beyotime)
99
Beyotime klenow fragment
A) Schematic of DNA construct preparation for single-molecule force spectroscopy. A ∼6.8 kb plasmid was linearized with the restriction enzyme NotI and the 5’-overhangs were biotinylated using <t>Klenow</t> <t>fragment.</t> The CpG methyltransferase (MTase) M.SssI was used to methylate all cytosines at the C-5 position (5mC) within the double-stranded CG dinucleotide sequence. B) Interpolation of force-extension data shows that the averaged force-distance (F,d) curves for unmethylated (−5mC) and methylated (+5mC) DNA follow different trajectories, reflecting variation in the mechanical properties upon CpG methylation. C ) CpG methylation significantly increases the DNA contour length (L C ) (Student’s t-test, p < 0.001, n = 34 and n = 26 for -5mC and +5mC, respectively). Box plots show quartiles; whiskers show the distribution excluding outliers. D ) CpG methylation significantly increases the DNA persistence length (L p ) from 47 ± 8 nm (unmethylated) to 52 ± 7 nm (methylated) (Student’s T-test, p < 0.05, n = 34 and n = 26 for -5mC and +5mC, respectively).
Klenow Fragment, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/klenow+fragment/Klenow+Fragment/pmc05588124-76-274-279
Average 99 stars, based on 1 article reviews
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large klenow fragment  (New England Biolabs)
99
New England Biolabs large klenow fragment
A) Schematic of DNA construct preparation for single-molecule force spectroscopy. A ∼6.8 kb plasmid was linearized with the restriction enzyme NotI and the 5’-overhangs were biotinylated using <t>Klenow</t> <t>fragment.</t> The CpG methyltransferase (MTase) M.SssI was used to methylate all cytosines at the C-5 position (5mC) within the double-stranded CG dinucleotide sequence. B) Interpolation of force-extension data shows that the averaged force-distance (F,d) curves for unmethylated (−5mC) and methylated (+5mC) DNA follow different trajectories, reflecting variation in the mechanical properties upon CpG methylation. C ) CpG methylation significantly increases the DNA contour length (L C ) (Student’s t-test, p < 0.001, n = 34 and n = 26 for -5mC and +5mC, respectively). Box plots show quartiles; whiskers show the distribution excluding outliers. D ) CpG methylation significantly increases the DNA persistence length (L p ) from 47 ± 8 nm (unmethylated) to 52 ± 7 nm (methylated) (Student’s T-test, p < 0.05, n = 34 and n = 26 for -5mC and +5mC, respectively).
Large Klenow Fragment, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/klenow+fragment/Klenow+Fragment/pm37316488-288-10-17
Average 99 stars, based on 1 article reviews
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dna polymerase i klenow fragment kit  (Vazyme Biotech Co)
93
Vazyme Biotech Co dna polymerase i klenow fragment kit
A) Schematic of DNA construct preparation for single-molecule force spectroscopy. A ∼6.8 kb plasmid was linearized with the restriction enzyme NotI and the 5’-overhangs were biotinylated using <t>Klenow</t> <t>fragment.</t> The CpG methyltransferase (MTase) M.SssI was used to methylate all cytosines at the C-5 position (5mC) within the double-stranded CG dinucleotide sequence. B) Interpolation of force-extension data shows that the averaged force-distance (F,d) curves for unmethylated (−5mC) and methylated (+5mC) DNA follow different trajectories, reflecting variation in the mechanical properties upon CpG methylation. C ) CpG methylation significantly increases the DNA contour length (L C ) (Student’s t-test, p < 0.001, n = 34 and n = 26 for -5mC and +5mC, respectively). Box plots show quartiles; whiskers show the distribution excluding outliers. D ) CpG methylation significantly increases the DNA persistence length (L p ) from 47 ± 8 nm (unmethylated) to 52 ± 7 nm (methylated) (Student’s T-test, p < 0.05, n = 34 and n = 26 for -5mC and +5mC, respectively).
Dna Polymerase I Klenow Fragment Kit, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/klenow+fragment/DNA+Polymerase+I+Klenow+Fragment/pmc05834795-101-17-23
Average 93 stars, based on 1 article reviews
dna polymerase i klenow fragment kit - by Bioz Stars, 2026-09
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klenow fragment and t4dnapol  (Promega)
90
Promega klenow fragment and t4dnapol
A) Schematic of DNA construct preparation for single-molecule force spectroscopy. A ∼6.8 kb plasmid was linearized with the restriction enzyme NotI and the 5’-overhangs were biotinylated using <t>Klenow</t> <t>fragment.</t> The CpG methyltransferase (MTase) M.SssI was used to methylate all cytosines at the C-5 position (5mC) within the double-stranded CG dinucleotide sequence. B) Interpolation of force-extension data shows that the averaged force-distance (F,d) curves for unmethylated (−5mC) and methylated (+5mC) DNA follow different trajectories, reflecting variation in the mechanical properties upon CpG methylation. C ) CpG methylation significantly increases the DNA contour length (L C ) (Student’s t-test, p < 0.001, n = 34 and n = 26 for -5mC and +5mC, respectively). Box plots show quartiles; whiskers show the distribution excluding outliers. D ) CpG methylation significantly increases the DNA persistence length (L p ) from 47 ± 8 nm (unmethylated) to 52 ± 7 nm (methylated) (Student’s T-test, p < 0.05, n = 34 and n = 26 for -5mC and +5mC, respectively).
Klenow Fragment And T4dnapol, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/klenow+fragment/klenow+fragment+and+t4dnapol/10__1074_slash_jbc__m101996200-141-52-53
Average 90 stars, based on 1 article reviews
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klenow dna polymerase end-filled bamhi/hindiii fragment from pabcluc  (Promega)
90
Promega klenow dna polymerase end-filled bamhi/hindiii fragment from pabcluc
A) Schematic of DNA construct preparation for single-molecule force spectroscopy. A ∼6.8 kb plasmid was linearized with the restriction enzyme NotI and the 5’-overhangs were biotinylated using <t>Klenow</t> <t>fragment.</t> The CpG methyltransferase (MTase) M.SssI was used to methylate all cytosines at the C-5 position (5mC) within the double-stranded CG dinucleotide sequence. B) Interpolation of force-extension data shows that the averaged force-distance (F,d) curves for unmethylated (−5mC) and methylated (+5mC) DNA follow different trajectories, reflecting variation in the mechanical properties upon CpG methylation. C ) CpG methylation significantly increases the DNA contour length (L C ) (Student’s t-test, p < 0.001, n = 34 and n = 26 for -5mC and +5mC, respectively). Box plots show quartiles; whiskers show the distribution excluding outliers. D ) CpG methylation significantly increases the DNA persistence length (L p ) from 47 ± 8 nm (unmethylated) to 52 ± 7 nm (methylated) (Student’s T-test, p < 0.05, n = 34 and n = 26 for -5mC and +5mC, respectively).
Klenow Dna Polymerase End Filled Bamhi/Hindiii Fragment From Pabcluc, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/klenow+fragment/klenow+dna+polymerase+end+filled+bamhi+hindiii+fragment+from+pabcluc/10__1074_slash_jbc__m305791200-69-11-21
Average 90 stars, based on 1 article reviews
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2 u tag dna polymerase  (Promega)
90
Promega 2 u tag dna polymerase
A) Schematic of DNA construct preparation for single-molecule force spectroscopy. A ∼6.8 kb plasmid was linearized with the restriction enzyme NotI and the 5’-overhangs were biotinylated using <t>Klenow</t> <t>fragment.</t> The CpG methyltransferase (MTase) M.SssI was used to methylate all cytosines at the C-5 position (5mC) within the double-stranded CG dinucleotide sequence. B) Interpolation of force-extension data shows that the averaged force-distance (F,d) curves for unmethylated (−5mC) and methylated (+5mC) DNA follow different trajectories, reflecting variation in the mechanical properties upon CpG methylation. C ) CpG methylation significantly increases the DNA contour length (L C ) (Student’s t-test, p < 0.001, n = 34 and n = 26 for -5mC and +5mC, respectively). Box plots show quartiles; whiskers show the distribution excluding outliers. D ) CpG methylation significantly increases the DNA persistence length (L p ) from 47 ± 8 nm (unmethylated) to 52 ± 7 nm (methylated) (Student’s T-test, p < 0.05, n = 34 and n = 26 for -5mC and +5mC, respectively).
2 U Tag Dna Polymerase, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/klenow+fragment/2+l+dna+polymerase+i+large++klenow++fragment++5+u++l+/10__1111_slash_j__1745___4573__2004__07504__x-46-46-54
Average 90 stars, based on 1 article reviews
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klenow fragment  (Kunkel GmbH)
90
Kunkel GmbH klenow fragment
A) Schematic of DNA construct preparation for single-molecule force spectroscopy. A ∼6.8 kb plasmid was linearized with the restriction enzyme NotI and the 5’-overhangs were biotinylated using <t>Klenow</t> <t>fragment.</t> The CpG methyltransferase (MTase) M.SssI was used to methylate all cytosines at the C-5 position (5mC) within the double-stranded CG dinucleotide sequence. B) Interpolation of force-extension data shows that the averaged force-distance (F,d) curves for unmethylated (−5mC) and methylated (+5mC) DNA follow different trajectories, reflecting variation in the mechanical properties upon CpG methylation. C ) CpG methylation significantly increases the DNA contour length (L C ) (Student’s t-test, p < 0.001, n = 34 and n = 26 for -5mC and +5mC, respectively). Box plots show quartiles; whiskers show the distribution excluding outliers. D ) CpG methylation significantly increases the DNA persistence length (L p ) from 47 ± 8 nm (unmethylated) to 52 ± 7 nm (methylated) (Student’s T-test, p < 0.05, n = 34 and n = 26 for -5mC and +5mC, respectively).
Klenow Fragment, supplied by Kunkel GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/klenow+fragment/klenow+fragment/pm08553554-51-6-19
Average 90 stars, based on 1 article reviews
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dna polymerase-l (klenow enzyme; large fragment)  (Promega)
90
Promega dna polymerase-l (klenow enzyme; large fragment)
A) Schematic of DNA construct preparation for single-molecule force spectroscopy. A ∼6.8 kb plasmid was linearized with the restriction enzyme NotI and the 5’-overhangs were biotinylated using <t>Klenow</t> <t>fragment.</t> The CpG methyltransferase (MTase) M.SssI was used to methylate all cytosines at the C-5 position (5mC) within the double-stranded CG dinucleotide sequence. B) Interpolation of force-extension data shows that the averaged force-distance (F,d) curves for unmethylated (−5mC) and methylated (+5mC) DNA follow different trajectories, reflecting variation in the mechanical properties upon CpG methylation. C ) CpG methylation significantly increases the DNA contour length (L C ) (Student’s t-test, p < 0.001, n = 34 and n = 26 for -5mC and +5mC, respectively). Box plots show quartiles; whiskers show the distribution excluding outliers. D ) CpG methylation significantly increases the DNA persistence length (L p ) from 47 ± 8 nm (unmethylated) to 52 ± 7 nm (methylated) (Student’s T-test, p < 0.05, n = 34 and n = 26 for -5mC and +5mC, respectively).
Dna Polymerase L (Klenow Enzyme; Large Fragment), supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/klenow+fragment/dna+polymerase+l++klenow+enzyme++large+fragment+/pm09058376-251-12-18
Average 90 stars, based on 1 article reviews
dna polymerase-l (klenow enzyme; large fragment) - by Bioz Stars, 2026-09
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large klenow fragment of dna polymerase i  (Promega)
90
Promega large klenow fragment of dna polymerase i
A) Schematic of DNA construct preparation for single-molecule force spectroscopy. A ∼6.8 kb plasmid was linearized with the restriction enzyme NotI and the 5’-overhangs were biotinylated using <t>Klenow</t> <t>fragment.</t> The CpG methyltransferase (MTase) M.SssI was used to methylate all cytosines at the C-5 position (5mC) within the double-stranded CG dinucleotide sequence. B) Interpolation of force-extension data shows that the averaged force-distance (F,d) curves for unmethylated (−5mC) and methylated (+5mC) DNA follow different trajectories, reflecting variation in the mechanical properties upon CpG methylation. C ) CpG methylation significantly increases the DNA contour length (L C ) (Student’s t-test, p < 0.001, n = 34 and n = 26 for -5mC and +5mC, respectively). Box plots show quartiles; whiskers show the distribution excluding outliers. D ) CpG methylation significantly increases the DNA persistence length (L p ) from 47 ± 8 nm (unmethylated) to 52 ± 7 nm (methylated) (Student’s T-test, p < 0.05, n = 34 and n = 26 for -5mC and +5mC, respectively).
Large Klenow Fragment Of Dna Polymerase I, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/klenow+fragment/klenow+fragment+of+dna+polymerase+i/10__1128_slash_aem__70__8__4711___4719__2004-96-44-47
Average 90 stars, based on 1 article reviews
large klenow fragment of dna polymerase i - by Bioz Stars, 2026-09
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Image Search Results


A) Schematic of DNA construct preparation for single-molecule force spectroscopy. A ∼6.8 kb plasmid was linearized with the restriction enzyme NotI and the 5’-overhangs were biotinylated using Klenow fragment. The CpG methyltransferase (MTase) M.SssI was used to methylate all cytosines at the C-5 position (5mC) within the double-stranded CG dinucleotide sequence. B) Interpolation of force-extension data shows that the averaged force-distance (F,d) curves for unmethylated (−5mC) and methylated (+5mC) DNA follow different trajectories, reflecting variation in the mechanical properties upon CpG methylation. C ) CpG methylation significantly increases the DNA contour length (L C ) (Student’s t-test, p < 0.001, n = 34 and n = 26 for -5mC and +5mC, respectively). Box plots show quartiles; whiskers show the distribution excluding outliers. D ) CpG methylation significantly increases the DNA persistence length (L p ) from 47 ± 8 nm (unmethylated) to 52 ± 7 nm (methylated) (Student’s T-test, p < 0.05, n = 34 and n = 26 for -5mC and +5mC, respectively).

Journal: bioRxiv

Article Title: Multiscale Physical Effects of CpG Methylation on DNA Mechanics, Nucleosome Wrapping, and Chromatin Condensates

doi: 10.64898/2026.04.13.715649

Figure Lengend Snippet: A) Schematic of DNA construct preparation for single-molecule force spectroscopy. A ∼6.8 kb plasmid was linearized with the restriction enzyme NotI and the 5’-overhangs were biotinylated using Klenow fragment. The CpG methyltransferase (MTase) M.SssI was used to methylate all cytosines at the C-5 position (5mC) within the double-stranded CG dinucleotide sequence. B) Interpolation of force-extension data shows that the averaged force-distance (F,d) curves for unmethylated (−5mC) and methylated (+5mC) DNA follow different trajectories, reflecting variation in the mechanical properties upon CpG methylation. C ) CpG methylation significantly increases the DNA contour length (L C ) (Student’s t-test, p < 0.001, n = 34 and n = 26 for -5mC and +5mC, respectively). Box plots show quartiles; whiskers show the distribution excluding outliers. D ) CpG methylation significantly increases the DNA persistence length (L p ) from 47 ± 8 nm (unmethylated) to 52 ± 7 nm (methylated) (Student’s T-test, p < 0.05, n = 34 and n = 26 for -5mC and +5mC, respectively).

Article Snippet: 5’oOverhangs were filled with dGTP and biotin-16-dCTP using Klenow fragment (Jena Bioscience).

Techniques: Construct, Force Spectroscopy, Plasmid Preparation, Sequencing, Methylation, CpG Methylation Assay